Collect. Czech. Chem. Commun. 1996, 61, 333-341

Polarographic Determination of 6-β-D-Glucopyranosyloxy-7-hydroxycoumarin

Jiří Bareka, Roman Hrnčířb, Josino C. Moreirac and Jiří Zimaa

a UNESCO Laboratory of Environmental Electrochemistry, Department of Analytical Chemistry, Charles University, 128 40 Prague 2, Czech Republic
b Severochema Cooperative, 461 71 Liberec, Czech Republic
c CESTEH/ENSP/FIOCRUZ, Rua Leopoldo Bulhoes 1480, Manguinhos, 210 41-210 Rio de Janeiro, Brazil


The polarographic behaviour was studied for 6-β-D-glucopyranosyloxy-7-hydroxycoumarin, a natural compound serving as an optical whitening agent. The substance can be quantitated by tast polarography, differential pulse polarography using a conventional dropping mercury electrode, and differential pulse polarography using a static mercury drop electrode over the regions of 20-1 000, 2-1 000, and 0.2-1 000 μmol l-1, respectively. The methods developed for the quantitation of the compound were applied to its direct determination in a raw product.

Keywords: Polarography; 6-β-D-Glucopyranosyloxy-7-hydroxycoumarin.