Collect. Czech. Chem. Commun. 1995, 60, 1573-1577

Lipase Immobilized by Sol-Gel Technique in Layers

Gabriela Kuncováa, Massimo Guglielmib, Pavel Dubinac and Bohuslav Šafářc

a Institute of Chemical Process Fundamentals, Academy of Sciences of the Czech Republic, 165 02 Prague 6-Suchdol, Czech Republic
b University of Padova, Via Marzolo 9, 35131 Padova, Italy
c Research Institute 070, 602 00 Brno, Czech Republic


Commercial lipase was immobilized into an organic-inorganic matrix formed by hydrolysis of silicon alkoxides (tetraethoxysilane, dimethyldiethoxysilane and methyltriethoxysilane) with (3-aminopropyl)triethoxysilane, (3-thiopropyl)trimethoxysilane and chlorodiisopropyloctylsilane. Hydrolytic activity of lipase was tested after addition of the enzyme to a prepolymer solution, after gelation, in xerogel particles and in thin layers deposited on glass slides by dip- or spin-coating. The prepolymer containing NH groups showed the higher activity then the native enzyme.