Collect. Czech. Chem. Commun. 1995, 60, 1986-1994

Self-Degradable Hydrogel with Covalently Bound Proteolytic Enzyme

František Rypáček and Václav Škarda

Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06 Prague 6, Czech Republic


Biodegradable hydrogel as a self-degradable system for controlled release of biologically active macromolecules was prepared from the biodegradable poly[N5-(2-hydroxyethyl)-L-glutamine-stat -N5-(2-methacryloyloxyethyl)-L-glutamine -stat-L-glutamic acid], to which a proteolytic enzyme, papain, was covalently bound. The polymer-enzyme conjugate was crosslinked by radical copolymerization with acrylamide. Significantly improved thermal stability and increased pH optimum of the catalytic activity of conjugated papain was observed. Self-degradation of the hydrogel by the action of encapsulated papain could be triggered by the addition of a low-molecular-weight enzyme activator, such as dithioerythritol.