Collect. Czech. Chem. Commun. 1994, 59, 330-335

Some Observations on Effect of Fluorescent Substances on Lucigenin Chemiluminescent Reaction

Viera Tomová and Peter Magdolen

Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, 833 34 Bratislava, Slovak Republic


The effect of several substances (e.g. fluorescein, eosin B, pyronin G, coumarin, glucose etc.) on the chemiluminescent reaction of lucigenin was examined. The best enhancer of the initial light intensity is eosin B (2.3 times). Fluorescein and pyronin G enhance the intensity of chemiluminescent reaction in time (t1/2 = 8.6 versus t1/2 = 7.49 min and t1/2 = 12.6 versus t1/2 = 7.09 min, respectively).