Collect. Czech. Chem. Commun. 1994, 59, 213-221
https://doi.org/10.1135/cccc19940213

Fluorescence Reagents for Labelling of Biomolecules. Part II. Reactions of 9-Isothiocyanatoacridine with Amino Acids

Dušan Podhradskýa, Peter Oraveca, Marián Antalíka and Pavol Kristianb

a Department of Biochemistry, P. J. Šafárik University, 041 54 Košice, Slovak Republic
b Department of Organic Chemistry, P. J. Šafárik University, 041 54 Košice, Slovak Republic

This article is part of serial Fluorescence Reagents for Labelling of Biomolecules.

Abstract

N-(9-Acridinylthiocarbamoyl)amino acids (ATC-AA) II - VII were synthesized by reaction of amino acids with 9-isothiocyanatoacridine I, a new fluorescence labelling agent. The amino acid derivatives II - VII show high relative fluorescence, which is suitable for the determination of nanomolar amounts of ATC-AA. The kinetic measurements show that reaction of I with amino acids is 6 to 22 times faster than analogous reaction of phenyl isothiocyanate. The possibility of using 9-isothiocyanatoacridine for structure determination of proteins is discussed.