Collect. Czech. Chem. Commun.
1993, 58, 1692-1698
https://doi.org/10.1135/cccc19931692
Synthesis of Oligodeoxynucleotides Eliminating the Use of Acetonitrile
Jiří Smrta, Luboš Arnolda, Jan Svobodab, Roman Hákb and Ivan Rosenberga
a Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 166 10 Prague 6, Czech Republic
b Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 166 10 Prague 6, Czech Republic
Abstract
H-Phosphonate synthesis of oligodeoxynucleotids is described in which acetonitrile is replaced by dichloromethane. This solvent is thereafter easily regenerated and reused. Practically "one solvent" procedure of oligonucleotide synthesis has been established. The preparation of 5'-O-(4,4'-dimethoxytriphenylmethyl)-N2-(N,N-dimethylaminomethylene)-2'-deoxyguanosine (Ic) and 5'-O-(4,4'-dimethoxytriphenylmethyl)-N6-(N,N-dimethylaminomethylene)-2'-deoxyadenosine (Id) is described.
