Collect. Czech. Chem. Commun. 1992, 57, 661-672

Metabolism of 1-(S)-(3-Hydroxy-2-phosphonomethoxypropyl)cytosine (HPMPC) in Human Embryonic Lung Cells

Tomas Cihlar, Ivan Votruba, Květoslava Horská, Radek Liboska, Ivan Rosenberg and Antonín Holý

Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6


The acyclic nucleotide analogue HPMPC is in human embryonic lung cells, cultured in vitro, transformed to its mono- and diphosphates (HPMPCp and HPMPCpp) and HPMPCp-choline; the synthesis of HPMPCp is catalysed by pyrimidine nucleoside monophosphate kinase (EC; HPMPCp-choline (analogue of CDP-choline) is formed from HPMPCpp and choline phosphate in the presence of CTP:phosphorylcholine cytidylyltransferase (EC These metabolites persist for a long time in the cellular pool even after HPMPC has been removed from the medium; on the other hand, they efflux to the medium. Neither HPMPC, nor any of its metabolites interfere with intracellular CDP-choline level.