Collect. Czech. Chem. Commun. 1992, 57, 2272-2278

Determination of Organic Bases by Ion-Pair Extraction Polarography Using Orange II as Counter Ion

Václav Koula, Daria Kučová and Jiří Gasparič

Department of Biophysics and Physical Chemistry, Faculty of Pharmacy, Charles University, 501 65 Hradec Králové


The combination of ion-pair extraction and differential pulse polarography is shown to be a method suitable for the determination of 10-7 mol l-1 concentrations of organic bases of quaternary ammonium compounds. Orange II (4-[2-hydroxy-1-naphtyl]azobenzenesulfonic acid) was found to be an appropriate polarographically active counter-ion. The proposed method was used for the determination of tetrapentylammonium bromide (as model compound), Septonex ([1-(ethoxycarbonyl)-pentadecyl]trimethylammonium bromide) and codeine.