Collect. Czech. Chem. Commun. 1991, 56, 1340-1347

3β,11α,17-Trihydroxy-5-pregnen-20-one 11-hemisuccinate as a haptene for RIA of "17α-hydroxypregnenolone"

Alexander Kasal, Jenny Pokorná and Jaroslav Zajíček

Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6


6β,17-Dihydroxy-3α,5-cyclo-5α-pregnan-20-one (VII) and 17-hydroxy-6β-methoxy-3α,5-cyclo-5α-pregnan-20-one (IX) were hydroxylated into the position 11α using Rhizopus nigricans fungi culture, and then selectively converted into haptene XIII for the RIA determination of 3β,17-dihydroxypregn-5-en-20-one (IV).