Collect. Czech. Chem. Commun. 1991, 56, 796-808

The liquid chromatography of cholesterol esters

Ladislav Svoboda and Alena Krejčová

Department of Inorganic Technology, Institute of Chemical Technology, 532 10 Pardubice


The retention characteristics were found for cholesterol and 19 of its esters with saturated and unsaturated carboxylic acids containing 1 to 22 carbon atoms using high performance liquid chromatography in the normal and reversed-phase systems. HPLC on silica gel in a mobile phase consisting of heptane and 2-propanol or ethyl acetate is especially suitable for analysis of the esters of the lower acids, while chromatography on an octadecyl silica gel sorbent in a water-tetrahydrofuran or acetonitrile-dichlormethane mobile phase can bee successfully used for the separation of esters with acids containing 10–22 carbon atoms, that can differ in the presence of double bonds in the acyl or through cis-trans isomerism.