Collect. Czech. Chem. Commun. 1990, 55, 2138-2145

Reversed-phase high performance liquid chromatographic behaviour of homologous series

Bohuslav Ritticha, Štěpánka Rudolfováb and Pavel Janderac

a Institute of Systematic and Ecological Biology, 603 00 Brno
b Research Institute of Animal Nutrition, 691 23 Pohořelice
c Institute of Chemical Technology, 532 10 Pardubice


The retention model for reversed-phase high-performance liquid chromatography (RP-HPLC) based on interaction indices was applied to separation of the homologous series members. The validity of the equations describing relationship between retention (log k) and the number of methylene groups in alkyl chain (Cn) and composition of mobile phase (φ) by using statistical methods was verified. The regression coefficients of a quadratic term for relationship log k vs Cn and log k vs φ can be neglected. The regression coefficient of interaction term Cnφ is statistically significant.