Collect. Czech. Chem. Commun. 1990, 55, 1372-1379

Isolation of rabbit insulin

Jiří Jiráčeka, Jana Barthováa and Tomislav Barthb

a Department of Biochemistry, Charles University, 128 40 Prague 2
b Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6


Insulin extract has been prepared from rabbit disintegrated pancreas by means of acidic ethanol extraction in combination with ammonium acetate precipitation and purified by permeation chromatography. The enriched preparation of rabbit insulin has been fractionated further by affinity chromatography on AH-Sepharose 4B substituted with polylysine.