Collect. Czech. Chem. Commun. 1990, 55, 1351-1365

On linear concentration dependences of K'm, V' and 1/νi enzyme kinetic parameters

Vladimír I. Krupyanko

Institute of Biochemistry and Physiology of Microorganisms, U.S.S.R. Academy of Sciences, Pushchino, Moscow Region, 142292, U.S.S.R.


The most frequently occurring incorrect uses of the plots of intercepts, slopes and other coordinates for the determination of constants of enzyme activation Ka and of nontrivial inhibition K(IIi), K(Vi), K(Ii), were analyzed. The correct plots are shown. Calculations of the K(Ii) and K(IIi) constants of nontrivial types of enzyme inhibition using the corrected plots of the intercepts are given.