Collect. Czech. Chem. Commun.
1990, 55, 1351-1365
https://doi.org/10.1135/cccc19901351
On linear concentration dependences of K'm, V' and 1/νi enzyme kinetic parameters
Vladimír I. Krupyanko
Institute of Biochemistry and Physiology of Microorganisms, U.S.S.R. Academy of Sciences, Pushchino, Moscow Region, 142292, U.S.S.R.
Abstract
The most frequently occurring incorrect uses of the plots of intercepts, slopes and other coordinates for the determination of constants of enzyme activation Ka and of nontrivial inhibition K(IIi), K(Vi), K(Ii), were analyzed. The correct plots are shown. Calculations of the K(Ii) and K(IIi) constants of nontrivial types of enzyme inhibition using the corrected plots of the intercepts are given.
