Collect. Czech. Chem. Commun. 1990, 55, 1321-1325

One step introduction of the activated carboxyl groups with long aliphatic arm onto hydroxyl groups containing polymers

Luboš Arnold and Jiří Smrt

Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6


Bis-N-hydroxysuccinimide ester of 1,10-decanediol bis-succinate (Ia) reacts with hydroxyl groups bearing polymers (Spheron, cellulose, Sephacryl) affording activated carboxyl group containing derivatives IIa. Analogous 4-nitrophenyl group bearing Spheron derivative IIb was prepared starting from bis-4-nitrophenyl ester of 1,10-decanediol bis-succinate (Ib). 3'-O-(4,4'-Dimethoxytriphenylmethyl)-2'-deoxythymidine 5'-(6-amino-1-hexyl)-phosphate (V) was developed as reagent to determine the extent of activated carboxyl groups. Attachment ranging from 3.1-7.3 μmol/g was achieved.