Collect. Czech. Chem. Commun. 1990, 55, 1119-1126

Generation of electronically excited states by means of methemoglobin and methemoglobin-haptoglobin complex

Hana Hájková and Zdeněk Pavlíček

Department of Physical Chemistry, Charles University, 128 42 Prague 2


It has been found that both human methemoglobin and its complex with human haptoglobin in their enzymatic action can generate products in electronically excited states. The time dependence has been measured of the chemiluminescence generated by both the haem proteins in their peroxidase as well as oxidase actions. The methemoglobin-haptoglobin complex exhibited, in its peroxidase action, a higher chemiluminescence as compared with methemoglobin alone. The chemiluminescence observed in the oxidase reaction was the same with both the haem proteins. The Stern-Volmer plots for quenching of chemiluminescence with indole and acrylamide have been constructed for both enzymatic reactions.