Collect. Czech. Chem. Commun. 1990, 55, 2781-2786

Regiospecific cleavage of RNA by RNase H from E. coli in the presence of a complementary oligonucleotide with inserted alternating 2'-O-methylcytidine residues

Valerii G. Meteleva, Natalya F. Krynetskayab, Andrei A. Purmalb, Zoya A. Shabarovab, Zdeněk Točíkc, Luboš Arnoldc and Jiří Smrtc

a A. N. Belozersky Laboratory, Moscow State University, Moscow 119 899, U.S.S.R.
b Chemical Department, Moscow State University, Moscow 119 899, U.S.S.R.
c Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6, Czechoslovakia


Undecanucleotide dA-dC-dC-dA-Cm-Cm-dG-Cm-dG-Cm-dT (Cm = 2'-O-methylcytidine) was prepared and used as a probe for splitting of 5S rRNA fragment by RNase H (E. coli). It was found that substitution of 2'-deoxycytidine of d(ACCACCGCGCT) by 2'-O-methylcytidine in positions 5, 6, 8 and 10 provided regiospecific splitting of RNA fragment by RNase H in the presence of modified probe between U25 and C26.