Collect. Czech. Chem. Commun. 1990, 55, 2629-2635

Interpretation of chromatographic characteristics of mobile phase in liquid chromatography by means of solvent parameters

Oldřich Pytelaa, Jaroslava Hálováb and Miroslav Ludwiga

a Department of Organic Chemistry, Institute of Chemical Technology, 532 10 Pardubice
b Institute of Inorganic Chemistry, Czechoslovak Academy of Sciences, 250 68 Řež


The paper applies the methods of correlation analysis and analysis of latant variables (PCA, PLS, canonical correlation) to study the interrelations of general chromatographic characteristics in the adsorption and partition chromatography0, P', χe, χd, χn, Iap) and interpretation posibilities of these characteristics by means of general solvent parameters. The analyzed set of chromatographical parameters has been found to be relatively heterogeneous, its dominant properties being expressible by two principal components including 75.6% of source variability. The chemometrical solvent scale with three parameters PAC, PBC, PPC proved suitable for interpretation of the chromatographic parameters. This scale in the PLS method with two necessary latent variables explains 64.9% of the variability of matrix of chromatographic characteristic and enables their description. An elution solvent series has been constructed on the basis of this chemometrical solvent scale by the method of multicriterial decision ELECTRA III.