Collect. Czech. Chem. Commun. 1989, 54, 2133-2140

A study of the separation of Mo(VI) and W(VI) by reversed phase HPLC

Milan Vrchlabský, Naděžda Pollaková and Aleš Hrdlička

Department of Analytical Chemistry, J. E. Purkyně University, 611 37 Brno


The RP HPLC separation of Mo(VI) and W(VI) in the form of their oxoanions or anionic chelates with 2,3-dihydroxynaphthalene (DHN) was studied using a 100 x 4 mm i.d. column packed with Silasorb C18 (10μm). The two elements were separated with a resolution of Rij = 1.8 in 10 min after injection of the DHN-derivatized anions into the stream (flow rate 1 ml min-1) of the mobile phase containing phosphate buffer (50 mmol l-1), DHN (0.5 mmol l-1) and tetrabutylammonium cations (1 mmol l-1) in 60% (v/v) methanol at pH 7.