Collect. Czech. Chem. Commun. 1989, 54, 2809-2813

Hydrolysis of olive oil by pancreatic lipase in biphasic organic-aqueous system

Vladimír Kérya, Jana Trančíkováa and Štefan Schmidtb

a Institute of Chemistry, Slovak Academy of Sciences, 842 38 Bratislava
b Slovak Technical University, Faculty of Chemical Technology, 812 37 Bratislava


Six ethylene oxide-based surfactants were tested for their suitability to mediate lipase action on olive oil in a water-organic biphasic system. In Slovasol EL only it was possible by lipase to catalyze hydrolysis of the substrate over a wide range of isooctane and water content in the system. The Michaelis-Menten parameters of lipase measured for this system are comparable with those measured in a reversed emulsion of water and oil-isooctane solution. The hydrolysis rate was not increased, however, in the presence of isooctane.