Collect. Czech. Chem. Commun. 1988, 53, 3067-3071

Isotachophoretic determination of soluble nitrates, nitriles, sulfates and phosphates in plant material

Igor Matejovič and Magdaléna Bieliková

Agrochemistry Laboratory, Research Institute of Plant Production, 921 68 Piešťany


Testing and statistical evaluation of six operating systems suitable for the isotachophoretic determination of NO3-, SO42-, NO2- and H2PO4-, with the aim of the determination of the mentioned anions in plant material, are described. The proposed sample pretreatment reflecting the effort to minimize the number of unavoidable steps consisted only of extraction and filtration. The attention was paid to quantitative aspects of the extraction and to the possibility of extracts contamination during filtration. Influence of the sample amount on the anion zone lengths and the reproducibility of the determination were investigated too.