Collect. Czech. Chem. Commun. 1987, 52, 1407-1415

Separation of the main components of raw acetonine by normal-phase liquid chromatography

Milena Vespalcová and Radim Vespalec

Institute of Analytical Chemistry, Czechoslovak Academy of Sciences, Leninova 82, 611 42 Brno


The separation procedure of raw acetonine, that is unstable at the room temperature, has been worked out. Alumina was used as the stationary phase. The mixture of an aliphatic hydrocarbon with an alcohol saturated with the concentrated aqueous solution of ammonium hydroxide was used as the mobile phase. It has been proved that the result of the analysis at the room temperature was not affected by the spontaneous decomposition of the sample provided that the analysis did not exceed 30 min. Raw acetonine was found to contain ten components, four of which were identified.