Collect. Czech. Chem. Commun. 1986, 51, 1795-1801

Interaction of human hemoglobin with oxygen in the presence of benzene and use of computer for construction of saturation curves

Hana Hájková, Zdeněk Pavlíček and Vítěz Kalous

Department of Physical Chemistry, Faculty of Natural Sciences, Charles University, 128 42 Prague 2


The time profile of changes in the oxygen saturation curves of human hemoglobin in the presence of benzene was investigated. The partial oxygen pressure p50, necessary for a half saturation, decreases during the first 3.5 h of interaction of hemoglobin with benzene and did not change afterwards. The character of changes in Hill's coefficient was similar. The oxygen saturation was modeled both for hemoglobin alone and, using MWC as a model of allosteric interaction, also for hemoglobin and benzene in a computer.