Collect. Czech. Chem. Commun. 1986, 51, 2722-2726

Improved separation of C12-C22 fatty acid phenacyl esters by reversed phase column liquid chromatography

Tomáš Haniš, Miroslav Smrž, Pavel Klír, Karel Macek and Zdeněk Deyl

Institute of Physiology, Czechoslovak Academy of Sciences, 142 20 Prague 4


Phenacyl esters of C12-C22 fatty acids were separated on Separon SGX C18 column, using a gradient elution with methanol-acetonitrile-water. The proposed gradient showed better resolution of the critical pairs C18:3-C14:0, C16:1-C20:4, and C16:0-C18:1 than the gradient elution with methanol-water or acetonitrile-water, or than the isocratic elution with methanol-acetonitrile-water. The optimum volume concentration (83%) of the sum of both methanol and acetonitrile was maintained constant for 35 min; in this period the acetonitrile concentration decreased linearly from the initial 42-60% to 0% while the methanol concentration increased from the initial 41-23% to 83% at the same rate. After 35 min the elution was completed with a methanol-water gradient. The whole analysis can be performed within 63 min at a flow rate 1 ml/min.