Collect. Czech. Chem. Commun. 1986, 51, 2091-2097

Protection, efficiency, and some practical aspects of use of CGC columns in high performance liquid chromatography

Ján Kehr and Mikuláš Chavko

Institute of Neurobiology, Slovak Academy of Sciences, 041 67 Košice


Some practical adaptations of compact glass cartridge (CGC) columns and design of related components of the high-performance liquid chromatographic system are suggested; included are the design of a Teflon guard column and its filling procedure, an intra-column sample injector for on-column sample delivery or for injection into the precolumn, replacement of a contaminated packing or replenishment of packing at the top of the CGC column, a procedure for shortening the column to a desired length, and the possibility of thermostatting the column by means of a water jacket. These adaptations make it possible to increase the lifetime of the CGC column as much as five times without loss of efficiency, and to use partly damaged or imperfectly packed columns.