Collect. Czech. Chem. Commun. 1985, 50, 1329-1334

Preparation and properties of three analogues of [5-leucine]enkephalin, substrates for enzyme conversion studies

Jaroslav Vičara, Linda Servítováb, Martin Flegelc, Karel Hauzerb and Tomislav Barthb

a Institute of Chemistry, Faculty of Medicine, Palacký University, 775 15 Olomouc
b Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6
c Léčiva, Modřany, 143 10 Prague 4


Analogues of [5-Leu]enkephalin, prolonged by methionine on the N-terminus or, by lysine or methionine on the C-terminus were prepared by fragment condensation, purified by ion exchange chromatography or high-pressure liquid chromatography. The substances were characterised by their opioid activity in a test on guinea-pig ileum in comparison with the activity of [5-Leu]enkephalin.