Collect. Czech. Chem. Commun. 1985, 50, 1110-1113

Isolation and the structure of the products of cholesterol biodegradation by the mutant Mycobacterium sp. CCM 3529

Růžena Míčková, Hana Hutlová, Pavel Pihera, Jiří Protiva and Vladimír Schwarz

Research Institute for Pharmacy and Biochemistry, 130 60 Prague 3


During the biodegradation of cholesterol by the mutant Mycobacterium sp. CCM 3529 1,4-androstadiene-3,17-dione (II) is formed as the main product. The following compounds were identified as characteristic by-products: a mixture of methyl esters IV and V in a 1 : 3 ratio, 4-androstene-3,17-dione (III), 22-hydroxy derivative I, 17β-hydroxy-4-androsten-3-one (XIII) and 17β-hydroxy-1,4-androstadien-3-one (XII).