Collect. Czech. Chem. Commun. 1985, 50, 978-983

Interaction of quaternary aromatic isoquinoline alkaloids with acetylcholinesterase from Electrophorus electricus

Jitka Ulrichováa, Jan Kovářb and Vilím Šimáneka

a Institute of Medical Chemistry, Medical Faculty, Palacký University, 775 15 Olomouc
b Institute of Biochemistry, Faculty of Natural Sciences, University J. E. Purkyně, 611 37 Brno


Fluorescence data for the interaction of acetylcholinesterase (EC, ACHE) from Electrophorus electricus with berberine, 13-ethylberberine, coralyne, and chelerythrine have been presented. By a comparison with known inhibitors of acetylcholinesterase, tetramethylammonium and tacrine, the electrostatic interaction of quaternary alkaloids with the γ-anionic site of enzyme could be confirmed. During the interaction, two molecules of the ligand are bound to the molecule of the enzyme.