Collect. Czech. Chem. Commun. 1985, 50, 978-983
https://doi.org/10.1135/cccc19850978

Interaction of quaternary aromatic isoquinoline alkaloids with acetylcholinesterase from Electrophorus electricus

Jitka Ulrichováa, Jan Kovářb and Vilím Šimáneka

a Institute of Medical Chemistry, Medical Faculty, Palacký University, 775 15 Olomouc
b Institute of Biochemistry, Faculty of Natural Sciences, University J. E. Purkyně, 611 37 Brno

Abstract

Fluorescence data for the interaction of acetylcholinesterase (EC 3.1.1.7, ACHE) from Electrophorus electricus with berberine, 13-ethylberberine, coralyne, and chelerythrine have been presented. By a comparison with known inhibitors of acetylcholinesterase, tetramethylammonium and tacrine, the electrostatic interaction of quaternary alkaloids with the γ-anionic site of enzyme could be confirmed. During the interaction, two molecules of the ligand are bound to the molecule of the enzyme.