Collect. Czech. Chem. Commun.
1985, 50, 2310-2318
https://doi.org/10.1135/cccc19852310
Applicability of glutaminyl-tRNA cyclotransferase in the peptide synthesis
Václav Čeřovský and Karel Jošt
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6
Abstract
Peptides with amino-terminal glutamine and their derivatives on treatment with crude papain preparation, containing glutaminyl-tRNA cyclotransferase (E.C. 2.3.2.5.), are converted into the corresponding compounds with pyroglutamic acid. This enzymic cyclization does not take place with glutamic acid or its γ-derivatives. The reaction has been optimized for the preparation of pyroglutamic acid phenylhydrazide. Glutaminyl-leucine phenylhydrazide and glutaminyl-histidyl-proline amide reacted to give pyroglutamyl-leucine phenylhydrazide and TRH, respectively. Treatment of crude papain with iodoacetamide completely inhibited its activity without affecting the glutaminyl-cyclotransferase activity.
