Collect. Czech. Chem. Commun. 1985, 50, 2221-2227

A contribution to the description of the retention mechanism of metal chelates during their reversed-phase chromatography

František Vláčil and Věra Hamplová

Department of Analytical Chemistry, Prague Institute of Chemical Technology, 166 28 Prague 6


Mercury(II) diethyldithiocarbamate was chromatographed on silica gel with chemically bonded octadecyl groups using aqueous mixtures with methanol, acetonitrile, dioxane, and tetrahydrofuran, respectively, as the mobile phase, and the dependence of logarithm of the capacity ratio (k) on the volume fraction of water in the binary mobile phase was examined. Also, the dependence of k on the liquid-liquid extraction distribution ratio in an aqueous methanol-n-alkane extraction system was investigated for Cu, Co, Ni, Pb, and Hg diethyldithiocarbamates. Both kinds of dependence are linear, which suggests that the retention of the electroneutral metal chelates during the reversed-phase liquid chromatography on a nonpolar chemically bonded phase is due to the solvophobic effect.