Collect. Czech. Chem. Commun.
1985, 50, 2201-2209
https://doi.org/10.1135/cccc19852201
Synthesis of oligodeoxyribonucleotides using in situ prepared nucleoside methyl phosphochloridite intermediates
Jiří Smrta, Hana Večerkováb, Jitka Forstováb, Zdeněk Hostomskýb and André Rosenthalc
a Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6, Czechoslovakia
b Institute of Molecular Genetics, Czechoslovak Academy of Sciences, 166 10 Prague 6, Czechoslovakia
c Zentralinstitut fur Molecularbiologie, Akademie der Wissenschaften der DDR, 1115 Berlin-Buch, DDR
Abstract
Synthesis of oligodeoxyribonucleotides on silica gel support using 5'-O-dimethoxytrityl-(N-acyl)-deoxyribonucleoside 3'-(O-methyl phosphochloridite) intermediates (I) is described. Solutions of these intermediates were prepared before use by mixing of 100 μmol l-1 solution of methyl phosphodichloridite in 1,2-dichloroethane with the same volume of 120 μmol l-1 solution of 5'-O-dimethoxytrityl-(N-acyl)-deoxyribonucleoside in 1,2-dichloroethane containing 1 mol l-1 of sym-collidine. A bench type synthesizer for this methodology is described.
