Collect. Czech. Chem. Commun. 1985, 50, 286-290

Effect of thuringiensis on the initiation of in vitro RNA synthesis

Květoslava Horská and Karel Šebesta

Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6


Thuringiensin, a structural analog of ATP, which inhibits the elongation phase of RNA synthesis catalyzed by DNA-dependent RNA polymerase, also inhibits the initiation phase of this reaction. During the latter thuringiensin is not incorporated into the first site of the chain synthesized but inhibits the formation of pApU from 5'-AMP and UTP. If ATP and UTP are used as substrates the amount of the dinucleotide, a product of abortive synthesis, increases at low thuringiensin concentrations. Both elongation and abortive synthesis are inhibited at higher concentrations of the inhibitor.