Collect. Czech. Chem. Commun. 1984, 49, 1974-1989

A supersensitive spectrophotometric method for the determination of uranium(VI) with eriochromazurol B in the presence of tensides

Milan Pištělkaa, Bohumil Stojekb and Josef Havelc

a Nuclear Power Station Dukovany, 675 50 Dukovany
b Odra River Basin, Water Management Enterprise, 701 26 Ostrava
c Department of Analytical Chemistry, Purkyně University, 611 37 Brno


The influence of various tensides on the sensitivity of the reaction of uranyl with chromatographically pure Eriochromazurol B (2'',6''-dichloro-3,3'-dimethyl-4-hydroxyfuchsone-5,5'-dicarboxylic acid) was studied and considerable bathochromic and hyperchromic effects were observed. The reaction is most sensitive in the presence of cetyltrimethylammonium or certylpyridinium bromide. Based on the study of the equlibria and optimization of the conditions, a method is suggested for the determination of uranium traces in waters consisting in the measurement of absorbance at 624 nm at pH 5. With the molar absorptivity value of 136 000, the method ranks among the most sensitive spectrophotometric methods of determination of uranium in general. The uranium traces in waters can conveniently be extracted with tri-n-octylamine and reextracted into an aqueous phase. For 10 ml samples, the detection limit is 0.025 ppm uranium. The results obtained are evaluated statistically.