Collect. Czech. Chem. Commun. 1984, 49, 764-771

Preparation of short glass microbore columns for liquid chromatography and their properties

Danuše Kouřilová, Karel Šlais and Miloš Krejčí

Institute of Analytical Chemistry, Czechoslovak Academy of Sciences, 611 42 Brno


The preparation is described of thick-walled glass columns, inner diameter 0.5 mm and outer diameter 4.5 mm, packed with Separon SI C 18 (dp = 6 and 10 μm) from suspension under pressures of 20-35 MPa in the presence of an ionogenic detergent; the column length ranged from 5 to 30 cm. The minimum value of the height equivalent to a theoretical plate was Hmin = 2.8 - 3.3dp. The effect of the extra-column contributions to the dispersion on the slope of the linear part of the dependence of the height equivalent to a theoretical plate on the mobile phase velocity was examined and found to become significant at higher velocities, thereby imposing limitations on the shortening of the time of analysis.