Collect. Czech. Chem. Commun. 1984, 49, 752-756

An enzyme electrode for determination of aromatic diamines and aminophenols based on cross-linked ceruloplasmin

Lumír Macholána and Milan Jílekb

a Department of Biochemistry, J. E. Purkyně University, 611 37 Brno
b Institute of Experimental Botany, Czechoslovak Academy of Sciences, 772 00 Olomouc


The determination of aromatic amines and phenols by an enzyme pO2 electrode containing cross-linked ceruloplasmin in the reaction layer was examined. The electrode shows at pH 5.5 a marked selectivity for derivatives with-NH2 or NR2, resp., and -OH-groups in para and ortho position while its sensitivity for aromatic diamines is better than its sensitivity for aminophenols and polyphenols. p-Phenylenediamine and its N,N-dialkyl derivatives are the best analytes which can be detected starting from concentrations of 10-5 mol l-1 and higher; the reproducibility is 5-7% at an electrode response time of 2-5 min.