Collect. Czech. Chem. Commun. 1984, 49, 474-480

Effect of reabsorption on the fluorescence spectra of chlorophyll-a

Marie Urbanováa, Jan Naušb, Jan Hálaa and Ludvík Parmaa

a Faculty of Mathematics and Physics, Charles University, 121 16 Prague 2
b Faculty of Natural Sciences, Palacký University, 771 46 Olomouc


Simple formula for the reabsorption correction was tested on the fluorescence spectra of thin polystyrenefoils with built-in chlorophyll-a. The spectra were studied at various pigment concentrations, wavelengths and angles of incidence of the exciting radiation. It is shown that neglecting the reabsorption effect in dependence of the experimental arrangement can lead to a strong distortion of the fluorescence spectrum. The derived formula yields a good qualitative picture of the fluorescence spectra.