Collect. Czech. Chem. Commun. 1984, 49, 444-454

Analysis of the experimental binding data obtained in the radioimmunoanalysis of thyroxine

Peter Talán and Juraj Mucha

Institute of Radioecology and Application of Nuclear Techniques, 040 61 Košice


The antithyroxine antiserum parameters, namely the association constant and the so-called binding capacity, have been determined in the radioimmunoanalysis of thyroxine using the Scatchard transformation with modified input parameters. Limitations of this method are demonstrated and a new transformation of the radioimmunoanalytical results is proposed, which takes into consideration the relation between the concentrations of antigen and the binding sites of the antibody. The applicability of the Scatchard relation can be decided using this transformation.