Collect. Czech. Chem. Commun. 1984, 49, 2713-2719

Biodegradation of cholesterol by a mutant of the Mycobacterium species

Vladimír Schwarz, Pavel Pihera, Jiří Protiva and Růžena Míčková

Research Institute for Pharmacy and Biochemistry, 194 04 Prague


The biodegradation of cholesterol by the Mycobacterium mutant CCM 3528 gave rise to 22-hydroxy-23,24-bisnorchola-1,4-diene-3-one (I) as the main product. The identified by-products were 24-norchola-1,4-diene-3,22-dione (IX), androsta-1,4-diene-3,17-dione (XV) and their 4-unsaturated analogues, X and XVI respectively.