Collect. Czech. Chem. Commun. 1984, 49, 165-169

The tyrosyl and tryptophyl residues of orosomucoid studied by difference and derivative spectrophotometry

Petr Kálal and Vítěz Kalous

Department of Physical Chemistry, Charles University, 128 40 Prague 2


The accessibility of the tyrosyl and tryptophyl residues in orosomucoid (an acid α1-glycoprotein) was studied by the methods of temperature-perturbation difference spectrophotometry spectrophotometry and derivative spectrophotometry. It has been found that four residues of tyrosine and one residue of tryptophan are on the surface of the molecule, exposed to the solution. The method of derivative spectrophotometry has been modified to be applicable to proteins containing both tyrosine and tryptophan.