Collect. Czech. Chem. Commun. 1983, 48, 2558-2568

Amino acid sequence of the acidic acrosin inhibitor (BUSI I B2) from bull seminal plasma

Bedřich Melouna, Věra Jonákováb and Dana Čechováb

a Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6
b Institute of Molecular Genetics, Czechoslovak Academy of Sciences, 166 10 Prague 6


The molecule of the inhibitor consists of 63 amino acid residues whose sequence is the following: Glu-Ile-Tyr-Phe-Glu-Pro-Asp-Phe-Gly-Phe-Pro-Pro-Asp-Cys-Lys-Val-Tyr-Thr-Glu-Ala-Cys-Thr-Arg-Glu-Tyr-Asn-Pro-Ile-Cys-Asp-Ser-Ala-Ala-Lys-Thr-Tyr-Ser-Asn-Glu-Cys-Thr-Phe-Cys-Asn-Glu-Lys-Met-Asn-Asn-Asp-Ala-Asp-Ile-His-Phe-Gln-His-Phe-Gly-Glu-Cys-Glu-Tyr. The sequential data were obtained by the analysis of peptides isolated from the tryptic and chymotryptic digest of the carboxymethylated inhibitor. The molecular weight of the inhibitor calculated from its amino acid sequence is 7377.