Synthesis of oligodeoxyribonucleotides from 3'-O-formyl-(N-acyl)-2'-deoxyribonucleosides using <i>o</i>-chlorophenylphosphoditriazolide and/or methyl phosphodichloridite. Removal of methyl group from phosphotriester by the action of aqueous pyridine

Večerková, Hana; Smrt, Jiří

doi:10.1135/cccc19831323

CCCC > Archive > 1983, Volume 48 > Issue 5 > p. 1323

Synthesis of oligodeoxyribonucleotides from 3'-O-formyl-(N-acyl)-2'-deoxyribonucleosides using o-chlorophenylphosphoditriazolide and/or methyl phosphodichloridite. Removal of methyl group from phosphotriester by the action of aqueous pyridine

Hana Večerková^a and Jiří Smrt^b

^a Institute of Molecular Genetics, Czechoslovak Academy of Sciences, 166 10 Prague 6
^b Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6

Abstract

d-GGAATTCC (XI) was prepared by phosphomonotriazolide method in solution. Synthesis of oligodeoxyribonucleotides by phosphite method in solution using methyl phosphodichloridite was studied. Preparation of P-methyl esters of protected d-CA (XVII), d-CAT (XIX), d-TT (XXI) and d-ATT (XXIII) is described. The removal of methyl group from methyl containing phosphotriester by the action of aqueous pyridine is described.

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Synthesis of oligodeoxyribonucleotides from 3'-O-formyl-(N-acyl)-2'-deoxyribonucleosides using o-chlorophenylphosphoditriazolide and/or methyl phosphodichloridite. Removal of methyl group from phosphotriester by the action of aqueous pyridine

Abstract