Collect. Czech. Chem. Commun. 1983, 48, 798-804

A biospecific membrane sensor for the determination of sucrose

Lumír Macholán and Hana Konečná

Department of Biochemistry, Purkyně University, 611 37 Brno


The paper describes a rapid method for the biospecific determination of sucrose using an oxygen electrode of the Clark type or a platinum disc anode, the measuring part of which is coated by a thin film of invertase, mutarotase and glucose oxidase co-crosslinked by glutardialdehyde together with serum albumin. After injecting the sample into the reaction vessel the current corresponding to the decrease of the oxygen content or to the formation of hydrogen peroxide in the enzyme reaction layer is registered. The steady state electrode response is proportional to the concentration of sucrose within the range of 0.03 to 1.5 mmol . l-1 and is attained during 1-2 minutes with a reproducibility of 3-4%.