Collect. Czech. Chem. Commun. 1983, 48, 672-678

A specific enzyme electrode for spermine and spermidine

Lumír Macholán and Dagmar Jílková

Department of Biochemisty, J. E. Purkyně University, 611 37 Brno


A sensor method has been worked out for the determination of spermine and spermidine in concentrations 0.02-0.5 mmol l-1. The method is based on amperometric measurement of the consumption of oxygen and the formation of hydrogen peroxide in a reaction catalyzed by crosslinked polyamine oxidase from maize. One determination takes 1 to 2 min, with phosphate at least 8 months. The optimum conditions for the analysis are described and the applicability of the sensor to urine samples is dealt with.