Collect. Czech. Chem. Commun. 1983, 48, 336-345

Reactions of 2-furylethylenes with thiols in vivo

Ernest Šturdík, Ľudovít Drobnica and Štefan Baláž

Department of Technical Microbiology and Biochemistry, Slovak Institute of Technology, 812 37 Bratislava


The reaction of furylethylenes with thiols proceeds also in vivo. This have been proved with Ehrlich ascitic carcinoma cells and yeast under steady-state conditions by determination of SH groups employing the Ellman reagent. 1-(5-Nitro-2-furyl)-2-nitroethylene caused a considerable decrease of intracellular thiols content proportional to the amount used. A correlation between the degree of modification of SH groups in vivo and the reactivity towards thiols estimated under the in vivo conditions was found. The ability of furylethylenes to react with SH groups is decisive for their biologic activity.