Collect. Czech. Chem. Commun. 1983, 48, 184-191

Immobilization of enzymes introducing spacers. A synthesis of carriers with spacers of various length

Christian Flemminga, Anton Gaberta, Helmut Wanda and Jiří Zemekb

a Institute of Technical Chemistry, Academy of Sciences of GDR, 705 Leipzig, German Democratic Republic
b Institute of Chemistry, Slovak Academy of Sciences, 842 38, Czechoslovakia


A synthesis of spacers of various length and type, utilizing α,ω-dicarboxylic acids, α,ω-diaminoalkanes and succinic anhydride, condensation with carbodiimide, or introduction of -NCS functional groups is described. Carriers with a spacer were prepared by a method of binding the already synthesized spacer to the carrier (glass), and alternatively, by a stepwise synthesis of the spacer on the carrier surface. Carriers containing functional groups (37.2 - 39.3 μmol/g - NCS and 25 - 41.5 μmol/g - COOH) prepared in this way had total length of the spacer 0.62 - 3.92 nm. Whereas the length of the spacer is if no substantial importance for the reaction with low-molecular substances (L-valine, L-cysteine and 2-mercaptoethanol), the optimum length of the spacer for high-molecular compounds (albumin) is about 1.75 - 2.05 nm. The hydroxyl group adjacent to the functional group of the spacer (1,3-diaminopropan-2-ol) is also of noticeable influence.