Collect. Czech. Chem. Commun. 1982, 47, 2814-2823

Sequential studies on peptides prepared by chymotryptic digestion of chicken pepsinogen and partial covalent structure of the protein

Miroslav Baudyš and Vladimír Kostka

Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6


The peptides obtained by chymotryptic digestion of the S-sulfo derivative of chicken pepsinogen and of the large tryptic fragments of the same derivative were subjected to sequential analysis. The results obtained afforded information on nonoverlapping sequences of 329 amino acid residues in the molecule of the zymogen and yielded data on links between individual fragments in the middle part of the chain obtained in earlier work. A partial covalent structure of the protein is presented which contains 356 amino acid residues in eight segments of uninterchangeable order.