Collect. Czech. Chem. Commun.
1981, 46, 48-51
https://doi.org/10.1135/cccc19810048
Gradual exposure of cystine side-chain residues during urea denaturation of human serum albumin
Josef Chmelík, Jiří Kadleček and Vítěz Kalous
Department of Physical Chemistry, Charles University, 128 40 Prague 2
Abstract
Showing the accessibility of disulfide groups in the protein molecule, the polarographic catalytic hydrogen current (Brdicka current) was employed for the investigation of the urea denaturation of human serum albumin. The stepwise character of the denaturation curve was accounted for the gradual conformational changes of the protein molecule and related increasing accessibility of cystine side-chain residues.
