Collect. Czech. Chem. Commun. 1981, 46, 48-51

Gradual exposure of cystine side-chain residues during urea denaturation of human serum albumin

Josef Chmelík, Jiří Kadleček and Vítěz Kalous

Department of Physical Chemistry, Charles University, 128 40 Prague 2


Showing the accessibility of disulfide groups in the protein molecule, the polarographic catalytic hydrogen current (Brdicka current) was employed for the investigation of the urea denaturation of human serum albumin. The stepwise character of the denaturation curve was accounted for the gradual conformational changes of the protein molecule and related increasing accessibility of cystine side-chain residues.