Collect. Czech. Chem. Commun. 1980, 45, 2029-2034

The effect of serum proteins on the activity of pancreatic lipase

Josef Rokos, Pavel Procházka and Miroslav Nohýnek

Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4


The whole serum, its protein fractions isolated electrophoretically, and the pure human and rabbit serum albumins inhibited hydrolysis of tributyrin by a glycerine extract of porcine pancreatic lipase or by human pancreatic juice. Out of the isolated fractions, an eluate corresponding to α-globulin had the strongest inhibitory effect. Under the given experimental conditions the inhibition by albumin depended on its concentration in the medium. Digestion of albumin by pepsin completely destroyed its inhibitory effect on the lipolytic reaction.