Collect. Czech. Chem. Commun. 1980, 45, 1137-1143

Covalent attachment of proteins to Spheron by means of benzoquinone

Nicolina Stambolievaa and Jaroslava Turkováb

a Institute of Organic Chemistry, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria
b Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6, Czechoslovakia


The dependence of the amount and the activity of coupled chymotrypsin and trypsin on the activation time and pH, coupling time and pH, enzyme concentration in the reaction mixture and the molar ratio CaCl2/trypsin concentration were investigated for case where enzymes are covalentlybound to the hydroxyalkyl methacrylate gel (Spheron) by means of benzoquinone.