Collect. Czech. Chem. Commun. 1980, 45, 772-776

Activation of vasopressin hormonogens by kidney aminoacylarylamidase; Study in vitro

Karel Hauzer, Tomislav Barth and Karel Jošt

Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6


Under conditions in vitro the mammalian kidney aminoacylarylamidase splits off the individual glycine residues from the hormonogens of vasopressin and its analogs of the triglycyl-vasopressin type (triglycyl[8-lysine]vasopressin, triglycyl[8-ornithine]vasopressin and des-glycineamide triglycyl[8-lysine]vasopressin) generating consequently the hormone or its analogues. The time course of the reaction was followed by the biological method (pressoric assy) and by determing the concentration of the liberated glycine. The generated hormone or its analogues are resistant to the action of the enzyme used.