Collect. Czech. Chem. Commun. 1979, 44, 1312-1317

Statistical accuracy test in measurements of circular dichroism

Petr Pančoška, Ivo Frič and Karel Bláha

Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6


A collection of one hundred circular dichroism measurements (in acetonitrile) of the model cyclohexapeptide cyclo(glycyl-L-phenylalanyl-L-leucyl-glycyl-L-leucyl-L-phenylalanyl) has been evaluated using standard statistical methods. The reproducibility of the experimental intensity values is discussed with respect to the sample concentration and the particular regions of the CD curve. The maximum probable error in the intensity reading varies with wavelength from ±20 to ±10 000 deg cm2 dmol-1 in the spectral range from 274 to 196 nm, i.e. by 5-15% of the observed intensity.